Causes And Effects Of Limk1 - 1300 Words

Results Expression of LIMK1 was up-regulated in TCGA breast cancer database The expression of LIMK1 in breast cancer was checked in oncomine website (https://www.oncomine.org/). The TCGA database results (Figure 1) showed that LIMK1 was high expressed in different breast cancer type, including intraductal cribriform breast adenocarcinoma, invasive breast carcinoma, invasive lobular breast carcinoma and mixed lobular and ductal breast carcinoma. These results indicated that LIMK1 could be an oncogene in breast cancer. Mir-138-5p targeted LIMK1 and inhibited the expression of LIMK1 Several databases indicated that miR-138-5p could target LIMK1, such as TargetScan, PicTar and miRwalk, to regulate LIMK1/CFL1 signal pathway to function as a†¦show more content†¦Western blot showed that miR-138-5p could also inhibit the expression of PCNA, which was thought to be a proliferation biological marker13 .These results showed that miR-138-5p inhibited the proliferation of breast cancer cells. Mir-138-5p inhibited migration and invasion of breast cancer cells As shown in Figure 5A, the wound healing assay showed that over-expression of miR-138-5p in MDA-MB-231 breast cancer cells healed more slowly compared to NC group. At 24 h, in miR-138-5p treatment group, there was still 50% space not migrated by MDA-MB-231 cells while in NC group, the MDA-MB-231 cells have already healed already. Besides, the transwell assay showed over-expression of miR-138-5p also decreased the migrating and invasive cells (Figure 5B and 5C). It is well known that MMP2 and MMP9 are usually used as indicators of metastasis because they are important for metastatic cancer cell invading the basement membrane14, 15.Therefore we chose MMP2 and MMP9 as the biomarkers to reflect migration and invasion of metastatic breast cancer cells. As shown in Figure 5C and 5D, we found that miR-138-5p could significantly inhibit the protein level of MMP2 and MMP9 both in MDA-MB-231 and HCC1937 two breast cancer cellines. These results showed that over-expression of miR-138-5p inhibit ed migration and invasion of breast cancer cells. Mir-138-5p arrested breast cancer cells into G0/G1 phase After over-expression of miR-138-5p, MDA-MB-231 cells wereShow MoreRelatedThe Discovery Of The Small Rna Lin 4 Essay2141 Words   |  9 PagesFenelon et al., 2011) and enhancement of memory on several behavioral tasks (Bredy et al., 2011). Due to the discovery of non-canonical functions for Dicer, studies using Dicer knockouts should be carefully interpreted and verified for non-specific effects (Fukagawa et al., 2004; Kanellopoulou et al., 2005; Shapiro et al., 2010; Babiarz et al., 2011; Kaneko et al., 2011). In accordance with loss of function studies, dysfunction of the miRNA machinery and proteins associated with it has been implicatedRead MoreA Genetic Review Of Schizophreni Research Progress Of Chromosomes 22 And 83307 Words   |  14 PagesMedications are most commonly prescribed are antipsychotics. These affect the release of neurotransmitters in the brain such as dopamine and serotonin, which are thought to control symptoms of schizophrenia (Mayo Clinic Staff). Medications can often cause side effects that render patients unwilling to continue taking them. In these cases, treatment has to be forced upon the patients. The goal in medication treatment is to control the signs of schizophrenia with as low a dosage as possible. Therapy is another